The Combination of Jicama (Pachyrizus erosus L.) and Tofu and Alternative Medium for the Growth of Escherichia coli Bacteria

Abstract

Bacterial growth media is necessary for bacterial culture in  laboratory. Bacterial growth media is sold commercially, but its distribution has not yet reached all regions.  Local raw materials that have potential to fertilize the growth of microorganisms are jicama (Pachyrhizus erosus L.) and tofu. Jicama (Pachyrhizus erosus L.) can be a source of carbohydrate nutrition and tofu as a source of protein for microbial growth. This study aims to examine the potential combination of jicama (Pachyrhizus erosus L.) and tofu as an alternative bacterial growth medium. This research is an experimental study with a descriptive approach that uses 3 tests.  The bacterial inoculation method used is the pour plate method. The results showed that the natural medium of jicama (Pachyrhizus erosus L.) and the natural medium of tofu cannot be used as an alternative growth medium for escherichia bacteria. Heating in the process of making the media activates flavonoids in jicama (Pachyrhizus erosus L.) which can inhibit the growth of bacteria. The process of making tofu uses vinegar so it is acidic and bacteria are intolerant of its pH. Different methods and procedures must be carried out to produce jicama extract (Pachyrhizus erosus L.) and other non-acidic sources of protein.