The Effect of L-Ascorbic Acid-2-Phosphate on Human Adipose-Derived Stem Cells Population Doubling Time and Angiogenic Potency

Abstract

Adipose-derived stem cells (ADSCs) have been developed as substitute cells for tissue engineering. However, in order to get a high number of cells, over-repassage leads the cells to become big in spread size indicating that senescence occurred. This study aimed to evaluate the potency of ascorbic acid to induce the proliferation and angiogenic potency of human ADSCs and inhibit the aging process of the cells. Human ADSCs were isolated by an enzymatic process. ADSCs were cultured in 10% fetal bovine serum (FBS) supplemented by ascorbic acid in various concentrations (1, 3, 6 µg/ml). The morphology, proliferation, population doubling time (PDT), and angiogenic potency were evaluated. Angiogenic potency analysis was performed using gene expression of vascular endothelial growth factor (VEGF) and its receptor (VEGFR). Ascorbic acid on 6 µg/mL suppressed the senescence proved by cell spread areas, growth curves, viability, PDT, and percentage of scratch width closure. No significant difference in vascular endothelial growth factor and its receptor expression to the control. However, the high concentration of ascorbic acid (6 µg/ml) expressed the highest VEGF and VEGFR. Our study showed that ADSCs with supplementation of ascorbic acid (6 µg/ml) promoted proliferation and angiogenic potency.