Potential of Methanol Extract From Rosella (Hibiscus Sabdariffa) as an Alternative Dye in Gram Staining

Authors

  • Mohammad Indra Pratama Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Rajun Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Faradilla Dahlan Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Nurul Anisa Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Indri Yudita Ningsih Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Khusnul Yumni Bulan Sari Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Sri Sela Pratiwi Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Niky Aulia Putri Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Teguh Pribadi Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Didimus Tanah Boleng Biology Education Department Faculty of Teacher Training and Education, Mulawarman University
  • Dora Dayu Rahma Turista Biology Education Department Faculty of Teacher Training and Education, Mulawarman University https://orcid.org/0000-0002-8560-4510

Keywords:

Methanol, Rosella, E. coli, Staphylococus, Klebsilla, Safranin

Abstract

Gram staining is one of the important steps to identify bacteria. This type of staining uses synthetic substances safranin and violet crystals which can result in environmental pollution. Rosella flowers (Hibiscus sabdariffa) contain natural dyestuffs, namely anthocyanins that have the potential to color bacterial cells. This study aims to find out whether rosella flower methanol extract (Hibiscus sabdariffa) can be used as a substitute for alternative dyestuffs in Gram staining. This study is an experimental study with a descriptive approach that uses three repetitions. The results showed that rosella flower extract (Hibiscus sabdariffa) cannot be used as an alternative to natural dyes for staining bacteria Staphylococcus aureus, Klebsiella Pneumonia and E. coli.  The bacterial cell wall has a complex layer so it requires a special type of dye to be able to penetrate it. Different materials need to be carried out so that they can be used as alternative dyes in gram staining.

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Published

2024-07-30

Issue

Vol. 2 No. 1 (2023): Toward Sustainable Tropical Environment For New Capital City of Indonesia

Section

Articles