Phytochemical Analysis of Ethanol Extract from Stingless Bee (Tetragonula laeviceps Smith) Honey and Its Anti-Acnes Activity
Abstract
Ethanol extract of stingless bee (Tetragonula laeviceps) honey were conducted for its phytochemical analysis and anti-acnes potential by using agar well diffusion and microdilution
methods to conduct diameter of inhibition zone and minimum inhibitory concentration (MIC) value against Propionibacterium acnes, respectively. The result of phytochemical analysis demonstrated that ethanol extract of the stingless bee honey contains secondary metabolites namely alkaloids, steroids, triterpenoids, phenolics, saponins, tannins and quinones. Moreover, the ethanol extract displayed anti-acnes activity with diameter inhibition zone of 17.3 mm against P. acnes while MIC value at 0.78 μg/mL.
References
Abd Jalil, M. A., Kasmuri, A. R., & Hadi, H. (2017). Stingless bee honey, the natural wound healer: A review. Skin Pharmacology and Physiology, 30(2), 66-75. https://doi.org/10.1159/000458416
Avila, S., Beux, M. R., Ribani, R. H., & Zambiazi, R. C. (2018). Stingless bee honey: Quality parameters, bioactive compounds, health-promotion properties and modification detection strategies. Trends in Food Science & Technology, 81, 37-50. https://doi.org/10.1016/j.tifs.2018.09.002
Ávila, S., Hornung, P. S., Teixeira, G. L., Malunga, L. N., Apea-Bah, F. B., Beux, M. R., ... & Ribani, R. H. (2019). Bioactive compounds and biological properties of Brazilian stingless bee honey have a strong relationship with the pollen floral origin. Food Research International, 123, 1-10. https://doi.org/10.1016/j.foodres.2019.01.068
Bankova, V., Popova, M., & Trusheva, B. (2014). Propolis volatile compounds: chemical diversity and biological activity: a review. Chemistry Central Journal, 8(1), 1-8. https://doi.org/10.1186/1752-153X-8-28
Biluca, F. C., Braghini, F., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Physicochemical profiles, minerals and bioactive compounds of stingless bee honey (Meliponinae). Journal of Food Composition and Analysis, 50, 61-69.
https://doi.org/10.1016/j.jfca.2016.05.007
Biluca, F. C., de Gois, J. S., Schulz, M., Braghini, F., Gonzaga, L. V., Maltez, H. F., ... & Fett, R. (2017). Phenolic compounds, antioxidant capacity and bioaccessibility of minerals of stingless bee honey (Meliponinae). Journal of Food Composition and Analysis, 63, 89-97. https://doi.org/10.1016/j.jfca.2017.07.039
Biluca, F. C., Bernal, J., Valverde, S., Ares, A. M., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2019). Determination of free amino acids in stingless bee (Meliponinae) honey. Food Analytical Methods, 12(4), 902-907. https://doi.org/10.1007/s12161-018- 01427-x
Biluca, F. C., da Silva, B., Caon, T., Mohr, E. T. B., Vieira, G. N., Gonzaga, L. V., ... & Costa, A. C. O. (2020). Investigation of phenolic compounds, antioxidant and antiinflammatory activities in stingless bee honey (Meliponinae). Food Research International, 129, 108756. https://doi.org/10.1016/j.foodres.2019.108756
Biluca, F. C., Braghini, F., de Campos Ferreira, G., Costa dos Santos, A., Helena Baggio Ribeiro, D., Valdemiro Gonzaga, Vitali, L., Amadeu, M.G., Oliveira Costa, A. C., & Fett, R. (2021). Physicochemical parameters, bioactive compounds, and antibacterial potential of stingless bee honey. Journal of Food Processing and Preservation, 45(2), e15127. https://doi.org/10.1111/jfpp.15127
Chanchao, C. (2009). Antimicrobial activity by Trigona laeviceps (stingless bee) honey from Thailand. Pak J Med Sci, 25(3), 364-369.
Chanchao, C. (2013). Bioactivity of Honey and Propolis of Tetragonula laeviceps in Thailand. In Pot-Honey (pp. 495-505). Springer, New York, NY.
Choi, J. S., Bae, H. J., Kim, S. J., & Choi, I. S. (2011). In vitro antibacterial and antiinflammatory properties of seaweed extracts against acne inducing bacteria, Propionibacterium acnes. Journal of environmental biology, 32 (3), 313 – 318.
Chuttong, B., Chanbang, Y., Sringarm, K., & Burgett, M. (2015). Effects of long term storage on stingless bee (Hymenoptera: Apidae: Meliponini) honey. Journal of Apicultural Research, 54(5), 441-451. https://doi.org/10.1080/00218839.2016.1186404
CLSI. 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Ninth Edition. Clinical and Laboratory Standards Institute, United State of America. 12-19.
Chuah, E. L., Zakaria, Z. A., Suhaili, Z., Bakar, S. A., & Desa, M. N. M. (2014). Antimicrobial activities of plant extracts against methicillin-susceptible and methicillin-resistant Staphylococcus aureus. Journal of Microbiology Research, 4(1), 6-13. https://doi.org/10.5923/j.microbiology.20140401.02
Khongkwanmueang, A., Nuyu, A., Straub, L., & Maitip, J. (2020). Physicochemical Profiles, Antioxidant and Antibacterial Capacity of Honey from Stingless Bee Tetragonula laeviceps Species Complex. In E3S Web of Conferences (Vol. 141, p. 03007). EDP Sciences.
Marliana S.D., Suryanti V., & Suyono. (2005). Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq.Swartz.) dalam Ekstrak Etanol. Biofarmasi, 3 (1), 26-31.
Muruke, M. H. (2014). Assessment of antioxidant properties of honeys from Tanzania. Journal of Biology, Agriculture and Healthcare, 4(27), 22-29.
Omar, S., Mat-Kamir, N.F., & Sanny, M. (2019). Antibacterial activity of Malaysian produced stingless-bee honey on wound pathogens. Journal of Sustainability Science and Management, 14 (3), 67-79.
Rao, P. V., Krishnan, K. T., Salleh, N., & Gan, S. H. (2016). Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Revista Brasileira de Farmacognosia, 26, 657-664. https://doi.org/10.1016/j.bjp.2016.01.012
Ruga, R., & Chavasiri, W. (2019). Enhancing Antibacterial Activity by Combination of Chloramphenicol with Constituents from Dracaena cochinchinensis (Lour.) SC Chen. Anti-Infective Agents, 17(1), 74-80. https://doi.org/10.2174/2211352516666180730115216
Sarker, S. D., Nahar, L., & Kumarasamy, Y. (2007). Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 42(4), 321-324. https://doi.org/10.1016/j.ymeth.2007.01.006
Suntiparapop, K., Prapaipong, P., & Chantawannakul, P. (2012). Chemical and biological properties of honey from Thai stingless bee (Tetragonula leaviceps). Journal of Apicultural Research, 51(1), 45-52. https://doi.org/10.3896/IBRA.1.51.1.06
Syafrizal., Ramadhan, R., Kusuma, I. W., Egra, S., Shimizu, K., Kanzaki, M., & Tangkearung, E. (2020). Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity, 21(10).
Webster, G. F. (1995). Inflammation in acne vulgaris. Journal of the American Academy of Dermatology, 33(2), 247-253. https://doi.org/10.1016/0190-9622(95)90243-0 Wild, S., Gojka, R., Anders, G., Richard, S., & Hilary, K. 2004. Global prevalence of Diabetes. Diabetes Care, 27 (5), 1047-1053.
