Phytochemical Analysis and Screening of Antibacterial Activity of White Pacing Flower (Costus Speciosus (J. Koening) Sm.)

Authors

  • Ersa Marina Rahmawati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Eva Marliana Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Ritbey Ruga Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University

Keywords:

Costus speciosus, Antibacterial, Minimum Inhibitory Concentration

Abstract

This study was conducted with the aim of identifying secondary metabolite compounds and antibacterial activity of white pacing flower extract (Costus speciosus (J. Koenig) Sm.). Phytochemical screening tests are performed qualitatively and antibacterial activity tests are carried out by agar well diffusion methods with modification against Staphylococcus aureus ATCC 25923, Propionibacterium acnes KCCM 41747, Streptococcus mutans ATCC 25175, Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422. The results of phytochemical screening showed that crude ethanol extract containing flavonoids, phenolics, and saponins, in the methanol:water fraction containing flavonoids, phenolics, and tannins while the n-hexane and ethyl acetate fractions containing steroid compounds. Antibacterial screening showed the highest antibacterial activity in the ethyl acetate fraction against P. acnes with an inhibition zone diameter of 11.3 mm and a minimum inhibitory concentration value of 0.25%.

Keywords: Costus speciosus, Antibacterial, Minimum Inhibitory Concentration.

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Published

2024-07-30

Issue

Vol. 2 No. 1 (2023): Toward Sustainable Tropical Environment For New Capital City of Indonesia

Section

Articles