Antagonistic Test of Dark Septate Endophyte (DSE) Against Colletotrichum gloeosporioides on Chili Plantation (Capsicum annuum)

Abstract

Dark Septate Endophyte (DSE) is an endophytic fungus with dark, septate hyphae and has the potential as a biocontrol agent in controlling pathogenic fungi in plants. The purpose of this study was to determine the antagonism of DSE fungi isolated from red chili roots against Colletotrichum gloeosporioides fungi in vitro. This research is descriptive exploratory and experimental. Three isolates of DSE fungus isolated from red chili roots were tested for antagonism against C. gloeosporioides in vitro using dual culture 1 and dual culture 2 tests. The dual culture 1 test was by growing the DSE fungus first before meeting C. gloeosporioides and the dual culture 2 test was by growing the DSE fungus and C. gloeosporioides directly on agar media. The percentage of inhibition was analyzed using the ANOVA test and Duncan's advanced test. The results showed the highest percentage of inhibition of C. gloeosporioides by isolate CA7 was 80.83% (dual culture 1) and CA1 isolate was 51.33% (dual culture 2). The results of the ANOVA analysis showed that there was an effect on the treatment performed (p<0.05). Thus, DSE fungus isolated from the roots of red chili has the potential to inhibit C. gloeosporioides fungus.